ORCID:   0000-0001-8087-766X

简介

博士后（Michigan   Technological University，USA, 2012.1-2013.7）

博士（东华大学, 环境工程专业，2006）

硕士（安徽师范大学，有机化学专业，2001）

学士（安徽师范大学，化学教育专业，1992）

研究兴趣

       免疫分析，抗原抗体制备，生物学效应研究

    环境监测与分析，食品检测新方法

    纳米药物载体定量与可视化

主持科研项目

   5. 复杂基质中蛋白质电晕-纳米颗粒的整体定量及免疫相容性研究（安徽省自然科学基金，2021-2023），项目负责人

  4. 基于免疫学的纳米颗粒的定量检测及生物学效应研究（国家自然科学基金，2012-2015），项目负责人

  3. 动物源性食品中环境激素的快速检测新方法的建立与应用（安徽省高校自然科学基金重点项目，2011-2013），项目负责人

  2. 酞酸酯类环境激素分子生物学检测新技术研究（安徽省自然科学基金项目，2006），项目负责人

  1. 生物大分子有机纳米荧光探针的制备及其应用（安徽省博士后科研经费资助项目, 2005），项目负责人

主持教研项目

    化学专业设计性实验的教学探索与实践（安徽省级教学研究重点项目，2010），项目负责人

讲授课程

 《分析化学》《仪器分析》《基础化学实验》《综合化学实验》《分析化学实验》等

获奖情况

   2008年获安徽师范大学教学优秀一等奖；2022年获安徽省教学成果奖特等奖（排名第三）

代表性论文

28. Li Wang, Lei Li, Wei Chen, Mingcui Zhang*. An Ultrasensitive Fluorescent Immunoassay Based on R-CDs@SiO₂ Nanoparticles for Detection of Cardiac Troponin I. Applied Biochemistry and Biotechnology, online. DOI:   https://doi.org/10.1007/s12010-026-05914-4

27. Lei Li, Jing Li, Weiya Pei, Xueqin Li, Xiaolong Zhu, Mengying Zhang, Shujun Wan, Min Zhong, Mingcui Zhang*,   Yingying Zhang*, Kun Lv*. Immunoaffinity-confined binary nanozyme rate sensor for sensitive detection of extracellular vesicles by enhanced mass transfer efficiency. Sensors and Actuators: B. Chemical, 2026,468, 140680. DOI: https://doi.org/10.1016/j.snb.2026.140680

26. Li Wang, Lei Li, Wei Chen, Mingcui Zhang*. Fluorescence nanozyme B-CDs@SiO₂@MnO₂ for   the dual-mode immunoassay of cardiac troponin I. Analytical and Bioanalytical Chemistry, 2026, 418, 1517–1529. DOI:   https://doi.org/10.1007/s00216-026-06313-8

25. Lei Li, Xin Du, Li Wang, Yu Gu, Jing   Li, Xiaolong Zhu, Xueqin Li, Yifan Gu, Min Zhong, Mingcui Zhang*,   Yingying Zhang*, Kun Lv*. Self-catalytic synthesis of nanodot assemblies with   tyramine memory effect for dual-channel immunoassay of exosomal cardiac   troponin I. Microchemical Journal, 2025, 219, 115783. DOI: 10.1016/j.microc.2025.115783

24. Qiuning Zhang, Lei Li, Qianqian Yang,   Wei Chen, Ziyuan Wang, Mingcui Zhang*. Quantitative Intracellular   Delivery of Anticancer Nanodrugs Via an Immunoassay Employing Pt-SiO₂   Janus-Peroxidase Nanozyme. Molecular Pharmaceutics, 2024, 21, 11, 5598-5606. DOI: 10.1021/acs.molpharmaceut.4c00552

23. Biru Chen, Lei Li, Qianqian Yang, Mingcui   Zhang*. Self-corrected dual-optical immunosensors using carbon dots@SiO₂@MnO₂   improving diethyl phthalate detection accuracy, Talanta, 2023, 261, 124652. DOI: 10.1016/j.talanta.2023.124652

22. Biru Chen, Lei Li, Qianqian Yang,   Bolin Liu, Yue Hu & Mingcui Zhang*. Fluorescence Signal   Amplification: Red Carbon Dots@SiO₂-Induced Ultra-sensitive   Immunoassay for Diethyl Phthalate, Journal of Fluorescence,   2023, 33, 487-495. DOI: 10.1007/s10895-022-03100-3

21. Biru Chen, Lei Li, Yue Hu, Bolin Liu,   Ming Guo, Qi Zhang, Qianqian Yang, Mingcui Zhang*, Fluorescence turn-on   immunoassay of endocrine diethyl phthalate in daily supplies using red   fluorescent carbon dots, Microchemical Journal, 2022, 178,   107350. DOI: 10.1016/j.microc.2022.107350

20. Lei Li, Qi Zhang, Biru Chen, Ming   Guo, Qianqian Yang, Yuzhong Zhang*, and Mingcui Zhang*. Nano-Bio   Interface-Guided Nanoparticle Protein Corona Antigen for Immunoassays and   Immunoimaging in a Complex Matrix, ACS Applied Bio Materials,   2022, 5, 841-852. DOI: 10.1021/acsabm.1c01231

19. Lei Li,   Biru Chen, Ming Guo, Qianqian Yang, Yuzhong Zhang*, and Mingcui Zhang*.   Platinum Janus Nanoparticles as Peroxidase Mimics for Catalytic Immunosorbent   Assay, ACS Applied Nano Materials, 2022, 5, 1397-1407.   DOI: 10.1021/acsanm.1c03944

18. Lei Li, Xi Yan, Meng Xia, Bi Shen,   Yiting Cao, Xiayu Wu, Jinwen Sun, Yue Zhang, and Mingcui Zhang*. Nanoparticle/Nanocarrier   Formulation as an Antigen: The Immunogenicity and Antigenicity of Itself, Molecular   Pharmaceutics, 2022, 19, 148-159. DOI:   10.1021/acs.molpharmaceut.1c00704

17. Lei Li, Mingcui Zhang*.   Development of immunoassays for the determination of phthalates. Food   and Agricultural Immunology, 2020, 31(1): 303-316. DOI:   10.1080/09540105.2020.1721439

16. Lei Li, Zhongyu Lian, Xi Yan, Meng   Xia, Mingcui Zhang*. An evaporation induced self-assembly approach to   prepare polymorphic carbon dot fluorescent nanoprobes for protein labeling, Chemical Communications, 2018,   54, 13123-13126.DOI: 10.1039/c8cc05860a

15. Mingcui Zhang*, Wentong Hong,   Xiayu Wu, Yue Zhang, Fengzhu Li and Su-Qing Zhao. A highly sensitive and   direct competitive enzyme-linked immunosorbent assay for the detection of   di-(2-ethylhexyl) phthalate (DEHP) in infant supplies. Analytical   Methods, 2015, 7(13), 5441-5446. DOI: 10.1039/C5AY00207A

14. Durga Pokharel, Suntara Fueangfung, Mingcui   Zhang, Shiyue Fang*. Peptide and peptide nucleic acid syntheses using a   DNA/RNA synthesizer. Biopolymers, 2014,102(6): 487–493. DOI:   10.1002/bip.22574

13. Mingcui Zhang, Durga Pokharel,   Shiyue Fang*. Purification of Synthetic Peptides Using a Catching Full-Length   Sequence by Polymerization Approach. Organic Letters, 2014,16   (5), 1290-1293. DOI: 10.1021/ol403426u

12. Mingcui Zhang*, Yue Wang,   Xiaona Yu, Yurong Hu & Shaohui Liu. Rapid monitoring of dicyclohexyl   phthalate in foods using the direct competitive ELISA. Food and   Agricultural Immunology, 2014, 25(2), 229-242.   DOI:10.1080/09540105.2013.768964

11. Mingcui Zhang*, Xiaona Yu, Yue   Wang, Yurong Hu, Shaohui Liu. A Highly Sensitive Indirect Competitive   Enzyme-linked Immunosorbent Assay (ic-ELISA) by Antigen-coated for Diethyl   Phthalate Analysis in Foods. Food Analytical Methods, 2013, 6:1223–1228.   DOI: 10.1007/s12161-012-9529-9

10. Mingcui Zhang*, Yurong Hu,   Shaohui Liu, Ying Cong, Bolin Liu, Lun Wang*. A highly sensitive   enzyme-linked immunosorbent assay for the detection of dipropyl phthalate in   plastic food contact materials. Food and Agricultural Immunology,   2013, 24:2, 165-177. DOI:10.1080/09540105.2012.670615

9. Mingcui Zhang*, Yurong Hu,   Shaohui Liu, Ying Cong,Bolin Liu, Lun Wang*.Rapid Monitoring of Dipropyl   phthalate in Food Samples Using a Chemiluminescent Enzyme Immunoassay. Food   Analytical Methods, 2012, 5:1105–1113. DOI: 10.1007/s12161-011-9346-6

8. Mingcui Zhang*,Shaohui Liu,   Huisheng Zhuang*,Yurong Hu. Determination of Dimethyl Phthalate in   Environment Water Samples by a Highly Sensitive Indirect Competitive ELISA. Applied   Biochemistry and Biotechnology, 2012, 166, 436-445. DOI:   10.1007/s12010-011-9439-0

7. Mingcui Zhang*, Bolin Liu, Ying   Cong, Shaohui Liu, Yurong Hu. Development of highly specific fluorescence   immunoassay and enzyme-linked immunosorbent assay for detection of dimethyl   phthalate in water samples. Food and Agricultural Immunology,   2011, 22(4), 297-309. DOI: 10.1080/09540105.2011.575124

6. Mingcui Zhang*, Ying Cong, Yali   Sheng, Bolin Liu. A direct competitive enzyme-linked immunosorbent assay by   antibody coated for diethyl phthalate analysis. Analytical Biochemistry,   2010, 406 (1), 24-28. DOI: 10.1016/j.ab.2010.06.040

5. Mingcui Zhang*, Yali Sheng. An   Indirect Competitive Fluorescence Immunoassay for Determination of   Dicyclohexyl Phthalate in Water Samples. Journal of Fluorescence,   2010, 20(6), 1167-1173. DOI: 10.1007/s10895-010-0663-8

4. Mingcui Zhang, Qionge Wang, Huisheng Zhuang*. Determination   of Dibutyl o-Phthalate by Antigen-Coated Competitive Fluorescence   Immunoassay. Analytical Letters, 2007, 40(1), 127-   137

3. Mingcui Zhang, Qionge Wang, Huisheng Zhuang*. A novel   competitive fluorescence immunoassay for the determination of dibutyl   phthalate. Analytical and Bioanalytical Chemistry, 2006,   386(5), 1401-1406

2. 张明翠, 庄惠生*, 郎庆. 环境激素邻苯二甲酸二丁酯免疫检测Ⅱ―抗体的制备. 应用化学, 2007,24(5): 530-533

1. 张明翠, 庄惠生*, 郎庆. 邻苯二甲酸二丁酯免疫检测Ⅰ：人工抗原的制备及表征.   应用化学, 2006,23(8): 850-853

授权及转让专利

18. 张明翠, 张秋宁, 李磊. 一种疏水性抗癌药物姜黄素的比色-荧光双模式测定方法，中国发明专利，专利号：ZL 202311836855.3

17. 张明翠, 杨倩倩. 一种形貌可控的单孔中空纳米药物载体及其制备方法和应用. 中国发明专利，专利号：ZL 202210702307.0

16. 张明翠, 连中宇.一种粒径均匀、尺寸可控的高分子纳米药物载体油胺枝接聚琥珀酰亚胺的制备方法. 中国发明专利，专利号：ZL 201910803215.X

15. 张明翠, 连中宇. 一种负载抗癌药物喜树碱的纳米药物载体的荧光免疫定量检测方法. 中国发明专利，专利号：ZL 201910773247.X

14. 张明翠, 李磊. 一种基于磁性荧光探针标记的荧光免疫吸附测定超痕量高分子纳米药物载体的方法. 中国发明专利，专利号：ZL 201810459487.8

13. 张明翠, 夏濛. 一种检测叶酸功能化二氧化硅靶向纳米药物载体的方法. 中国发明专利, 专利号：ZL 201711150940.9

12. 张明翠, 闫希. 一种PSI-OAm-NAPI两亲聚合纳米材料的定量检测方法      发明. 中国发明专利, 专利号：ZL 201710489336.2

11. 张明翠, 夏濛. 一种叶酸功能化二氧化硅靶向纳米药物载体的定量检测方法. 中国发明专利, 专利号：ZL 201710489323.5

10. 张明翠, 夏濛. 一种基于直接竞争荧光免疫法定量检测叶酸功能化二氧化硅靶向纳米药物载体的方法. 中国发明专利, 专利号：ZL201710489337.7（已转让）

9. 张明翠, 闫希. 一种基于痕量荧光免疫定量检测PSI-OAm-NAPI两亲聚合纳米药物载体的方法. 中国发明专利, 专利号：ZL 201710489326.9

8. 张明翠, 沈碧. 一种氧化葡萄糖包被的四氧化三铁磁性纳米材料的定量方法. 中国发明专利, 专利号：ZL 201710044829.5

7. 张明翠, 曹以婷. 一种基于间接竞争酶联免疫法定量检测油胺枝接聚琥珀酰亚胺高分子纳米药物载体的方法. 中国发明专利, 专利号：ZL 201710051804.8

6. 张明翠, 张月. 一种基于间接竞争酶联免疫法定量检测80nmAuNPs的方法. 中国发明专利, 专利号：ZL 201510567972.3（已转让）

5. 张明翠, 吴瑕玉. 一种50nm银粒子的定量检测方法. 中国发明专利, 专利号：ZL 201410513013.9

4. 张明翠, 洪文童. 一种邻苯二甲酸丁基苄酯半抗原衍生物及制备方法、邻苯二甲酸丁基苄酯的检测方法. 中国发明专利, 专利号：ZL 201410512584.0（已转让）

3. 张明翠, 洪文童. 一种邻苯二甲酸丁基苄酯半抗原衍生物及制备方法. 中国发明专利, 专利号：ZL 201410512359.7

2. 张明翠，钟君亚. 邻苯二甲酸（2-乙基己）酯的间接竞争酶联免疫法检测方法. 中国发明专利, 专利号：ZL 201310675076.X（已转让）

1. 张明翠, 孙锦文. 一种测试痕量特定粒径纳米金粒子的方法. 中国发明专利, 专利号：ZL 201310722275.1

论 著

    教育部“十五”国家级规划教材, 化学实验(上). 北京：高等教育出版社出版(ISBN:7-04-011969-2), 2003年, 参编；第二版（2015年），副主编